Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® p21 Waf1/Cip1 siRNA II (+), using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 and α-Tubulin (11H10) Rabbit mAb #2125. The p21 Waf1/Cip1 (12D1) Rabbit mAb confirms silencing of p21 Waf1/Cip1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p21 Waf1/Cip1 siRNA.
Immunohistochemical analysis of paraffin-embedded HeLa cells, transfected with SignalSilence ® Control siRNA (Unconjugated) #6568 (upper) or SignalSilence ® p21 Waf1/Cip1 siRNA II #6558 (lower) using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.